Hitachi NanoFrontier LD LIT-TOF LCMS System

Hitachi Updated: 2008-09-03 RSS
Hitachi NanoFrontier LD LIT-TOF LCMS System

An Integrated NanoLC and LIT-TOF System — Innovative Technology Dedicated for the Analysis of Proteins

The highly efficient NanoFrontier is designed to meet the demanding challenges of today's proteomics research laboratories. The hybrid mass spectrometer incorporates Linear Ion Trap technology with TOF detection (LIT-TOF) affording superior sensitivity and mass accuracy.

MSn analysis using the LIT enables automatic MS/MS with automatic peak selection and exclusion of monovalent ions. This is useful for detecting post-translation modifications such as phosphorylation and glycosylation sites.

The NanoFrontier LC/MS utilizes the Hitachi NanoFrontier nLC chromatography system with DEGS (Dual Exchange Gradient System, patent pending) technology for unparalleled gradient and retention time reproducibility at true nanoflow (50nL/min).

The Hitachi IBA (Information Based Acquisition) intelligent MS/MS provides enhanced protein information at reduced analysis time.

NanoFrontier Mass Spectrometer Specifications
MS mode measurement range: 50 to 25,000 (m/z)
Precursor ion selection range: 150 to 4,000 (m/z)
Resolution: 10,000 (m/z 1,000) or over
MS accuracy: 5 ppm with internal reference
Nitrogen gas: Pressure 0.65 MPa; Max. flow rate 2.5 L/min; Purity 99% (with Nitrogen gas generator) or 99.95% (with Nitrogen gas cylinder)
Helium gas: Pressure 0.35 MPa; Max flow rate 10 mL/min; Purity 99.9999%
Weight: 925 lb
Dimensions: 47 in (w) x 32 in (d) x 55 in (h)
Power requirement: Single-phase 200V x 20A
Ambient temperature: 22 to 27 ˚C (-/+ 2 ˚C within a day)

NanoFrontier nLC Specifications
Max. pressure: 20 MPa
Flow rate range: 10 nL/min to 250 nL/min (gradient)
10 nL/min to 1,000 nL/min (conditioning)
Retention time reproducibility: RSD 0.5%
(Retention time of flow rate 200 nL/min)
Sample injection: (1) Autosampler: standard rack (200 vials) or optional 96-well plate rack; direct injection with volume 0.1 to 50¼; sample coolar to 4 degree C
(2) Manual injector (optional)
Dimension/weight: (w/ autosampler) W720 x L470 x H650 mm (85 kg)
(w/o autosampler) W340 x L470 x H650 mm (46 kg)
Power requirement: AC 100~240V ± 10%,50/60Hz
Ambient conditions: Humidity: 30~80%
Temperature: 15~30 degree C